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Advanced Characterization of ProteinsSeminar Topic: Advanced Characterization of Proteins by Dynamic Light Scattering (DLS), Multi-Angle Light Scattering (MALS) and Field-Flow Fractionation (AF4)
The seminar is addressed to all scientists who are interested in protein separation and characterization, aggregate detection, automated screening of proteins, protein-protein interactions, protein mobility, zeta potential, theory and practice of measuring absolute molar masses, molecular size/ shape and polydispersity of proteins and biomolecules in solution.
Dates & Locations:
- Monday, April 12th, 2010 in Brussels, Belgium (spaces available)
- Tuesday, April 13th, 2010 in Leiden, Netherlands (register now to be put on the waiting list)
- Wednesday, April 14th, 2010 in Berlin, Germany (spaces available)
- Thursday, April 15th, 2010 Søborg, Denmark (register now to be put on the waiting list)
- Friday, April 16th, 2010 in Uppsala, Sweden (spaces available)
- Monday, April 19th, 2010 in Basel, Switzerland at company Novartis Pharma AG
(spaces available) - Tuesday, April 20th, 2010 in Basel, Switzerland at company F. Hoffmann - La Roche AG
(spaces available) - Wednesday, April 21st, 2010 in Zurich, Switzerland (spaces available)
- Thursday, April 22nd, 2010 in Munich, Germany (spaces available)
Agenda:
Dynamic light scattering will be shown as a fast technique which allows measuring the hydrodynamic radius of the sample without dilution and with no separation. Special focus will be on the application of automated dynamic light scattering (DynaPro™ Plate Reader) in the research of proteins and various protein related materials.
The attendees will learn how Multi-Angle Light Scattering measurements can easily provide absolute molar mass and size without any calibration or using reference standards. Light scattering will be introduced as a method that can be used in a stand-alone mode or in combination with various separation techniques, such as size exclusion chromatography (SEC/GPC), HPLC or Field-Flow-Fractionation (FFF or AF4). Current users of conventional SEC will see that a light scattering detector, such as Wyatt's miniDAWN TREOS™, effectively solves the crucial problem of SEC, i.e., the column calibration, and provides much additional information.
Wyatt's Eclipse Field-Flow-Fractionation will be shown as an alternative separation technique for proteins with numerous advantages over traditionally used SEC.
Further highlights will be Composition Gradient Multi Angle Light Scattering (CG-MALS) to study protein interactions using Wyatt's Calypso™ instrument, as well as Massively Parallel Phase Analysis Light Scattering (MP-PALS) to study protein mobility and zeta potential using Wyatt's brand-new Möbiuζ™ Mobility instrument.
Seminar Program:
10:00 to 10:15 Welcome Address and Introduction
10:15 to 11:00 Lecture 1: "Basics of Light Scattering for Absolute Molar Mass Determination of Proteins and their Aggregates"
11:00 to 11:30 Coffee Break
11:30 to 12:00 Lecture 2: “Field-Flow-Fractionation (AF4): A Powerful Tool for Separation and Characterization of Proteins and Biomolecules”
12:00 to 13:00 Lunch
13:00 to 14:00 Lecture 3: "Dynamic Light Scattering and MP-PALS: Method, Instrumentation and Applications"
14:00 to 14:15 Coffee Break and Discussion
14:15 to 15:00 Lecture 4: Guest Lecture by Guest Speaker*
15:00 to 15:30 Closing Discussions
*Guest Speakers:
- in Brussels, Belgium: Prof. Dr. Kerensa Broersen, Free University Brussels
- in Leiden, Netherlands: Prof. Dr. Dirk Slotboom, University of Groningen
- in Berlin, Germany: Dr. Thorsten Lührs, Helmholtz Centre for Infection Research, Division of Structural Biology
- in Søborg, Denmark: Prof. Dr. Erik Huusfeldt Larsen, Technical University of Denmark and Dr. Per-Olof Wahlund, Novo Nordisk A/S
- in Basel, Switzerland: Dr. Johannes Ottl, Novartis Pharma AG
- in Basel, Switzerland: Dr. Hans-Joachim Schönfeld, F. Hoffmann - La Roche AG
- in Zurich, Switzerland: Dr. Lars Prade, Actelion Pharmaceuticals Ltd.
- in Munich, Germany: Frank Schaubhut, Coriolis PharmaService GmbH
and Dr. Axel Parbel, Modality Manager, GE Healthcare Life Sciences
Our Expert Speakers:
Registration:
The seminar is free-of-charge and has limited seating, so make your reservation early! Please use our online registration form to reserve your seat now!
 Register Now
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